Differential plating medium for quantitative detection of histamine-producing bacteria

Real-time polymerase chain reaction for quantitative detection of histamine-producing bacteria: use in cheese production.

Biogenic amines are toxic substances that appear in foods and beverages as a result of AA decarboxylation. The enzyme histidine decarboxylase catalyzes the decarboxylation of histidine to histamine, the biogenic amine most frequently involved in food poisoning. The aim of the present work was to develop a real-time quantitative PCR assay for the direct detection and quantification of histamine-...

Improved medium for detecting deoxyribonuclease-producing bacteria.

Incorporation of methyl green dye into an agar medium containing deoxyribo-nucleic acid results in an improved medium for detecting deoxyribonuclease-producing bacteria. Use of the dye makes it unnecessary to use acid to demonstrate deoxyribonuclease activity, thus allowing subculture or reincubation of colonies. The improved medium is highly sensitive and can be used for primary isolation of S...

Tellurite-glycine agar: a selective plating medium for the quantitative detection of coagulase-positive staphylococci.

The coagulae-positive staphylococci comprise a very homogeneous, readily identifiable species (Evans and Niven, 1950; Shaw et al., 1951) that includes all of the proven pathogenic and enterotoxin-producing strains of staphylococci. As a result of the clinical and public health significance of these organisms, a selective medium for their isolation and enumeration would have considerable value. ...

Detection of gram-negative histamine-producing bacteria in fish: a comparative study.

Poisoning due to ingestion of foods with elevated levels of biogenic amines (histamine, putrescine, cadaverine, and tyramine) is well documented. Histamine fish poisoning largely is due to growth of naturally occurring bacteria associated with scombroid fish species. A rapid and reliable method is needed to screen for the presence of histamine-forming bacteria in fish. This study included a com...

Primary plating medium for differentiating the colonies of the genus Proteus from certain other enteric bacteria.